Different techniques including toluene-ethanol, guanidine hydrochloride, guanidine hydrochloride-EDTA, lysozyme, lysozyme-EDTA and sonication treatments were used to extract penicillin G acylase from Escherichia coli ATCC 11105 cells. The obtained results show that penicillin G acylase extraction by
โฆ LIBER โฆ
The hydrolysis of cephalosporin G by free and immobilized penicillin G acylsse from a mutant of Escherichia coli ATCC 11105
โ Scribed by Erarslan, Altan
- Book ID
- 120427231
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 725 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1873-3298
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## Abstract Penicillin G amidase (PGA) is a key enzyme for the industrial production of penicillin G derivatives used in therapeutics. __Escherichia coli__ ATCC 11105 is the more commonly used strain for PGA production. To improve enzyme yield, we constructed various recombinant __E. coli__ HB101 a
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