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Influence of polyhydric compounds on the pH stability of penicillin G acylase obtained from a mutant of Escherichia coli ATCC 11105

โœ Scribed by Dilek Kazan; Altan Erarslan

Book ID
114080670
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
550 KB
Volume
31
Category
Article
ISSN
1873-3298

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Isolation of penicillin G acylase from E
Isolation of penicillin G acylase from Escherichia coli ATCC 11105 by physical and chemical treatments
โœ A Kheirolomoom; M Ardjmand; H Fazelinia; A Zakeri ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 151 KB

Different techniques including toluene-ethanol, guanidine hydrochloride, guanidine hydrochloride-EDTA, lysozyme, lysozyme-EDTA and sonication treatments were used to extract penicillin G acylase from Escherichia coli ATCC 11105 cells. The obtained results show that penicillin G acylase extraction by