Thermostabilization of penicillin G acylase obtained from a mutant of Escherichia coli ATCC 11105 by bisimidoesters as homobifunctional cross-linking agents

Isolation of penicillin G acylase from E

Isolation of penicillin G acylase from Escherichia coli ATCC 11105 by physical and chemical treatments

✍ A Kheirolomoom; M Ardjmand; H Fazelinia; A Zakeri 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 151 KB

Different techniques including toluene-ethanol, guanidine hydrochloride, guanidine hydrochloride-EDTA, lysozyme, lysozyme-EDTA and sonication treatments were used to extract penicillin G acylase from Escherichia coli ATCC 11105 cells. The obtained results show that penicillin G acylase extraction by