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The effect of pH on the transformation of syringic and vanillic acids by the laccases ofRhizoctonia praticolaandTrametes versicolor

✍ Scribed by A. Leonowicz; R. U. Edgehill; J.-M. Bollag

Publisher
Springer
Year
1984
Tongue
English
Weight
666 KB
Volume
137
Category
Article
ISSN
0302-8933

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✦ Synopsis

Laccases (benzenediol: oxygen oxidoreductases, EC 1.10.3.2) from Rhizoctonia praticola and Trametes versicolor formed different products from syringic and vanillic acids at different pH values, but both enzymes generated the same chemicals at a particular pH. The products were separated by thin-layer and high-performance liquid chromatography. Four compounds were determined from syringic acid (m/z 168, 334, 350 and 486) at pH 6.9, but only two of these (m/z 168 and 334) were found at pH 3.5. In the case of vanillic acid two isomeric dimers (m/z 304) and 2-methoxy-l,4-benzoquinone (m/z 138) were identified. The dimers were formed at both pH values (pH 3.5 and 6.9), but 2-methoxy-l,4-benzoquinone appeared only at pH 3.5.

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