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The effect of carbohydrate carbon sources on the production of constitutive and inducible laccases by Botryosphaeria sp.

✍ Scribed by Mário A. Alves da Cunha; Aneli M. Barbosa; Ellen C. Giese; Robert F. H. Dekker


Publisher
John Wiley and Sons
Year
2003
Tongue
English
Weight
77 KB
Volume
43
Category
Article
ISSN
0233-111X

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✦ Synopsis


Abstract

The influence of carbohydrates: glucose, fructose, galactose, galacturonic acid, xylose, lactose, sucrose, pectin and inulin, were evaluated as sole carbon source for the production of laccases by the ascomycete, Botryosphaeria sp. Veratryl alcohol, a laccase inducer, was added to culture media to study inducible laccase production on the same carbon sources. Inulinase and pectinase were also produced when Botryosphaeria sp. was grown on inulin, and galacturonic acid and pectin, respectively, and their levels were less in the presence of veratryl alcohol. Botryosphaeria sp. produced constitutive laccases on all carbon sources examined, and veratryl alcohol increased the laccase production on most of carbon sources studied except for inulin and galacturonic acid. Evidence is presented that Botryosphaeria sp. is also pectinolytic.


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