Growth and production of laccases by the ligninolytic fungi, Pleurotus ostreatus and Botryosphaeria rhodina , cultured on basal medium containing the herbicide, Scepter® (imazaquin)
✍ Scribed by Maria I. Rezende; Aneli M. Barbosa; Ana-Flora D. Vasconcelos; Renata Haddad; Robert F. H. Dekker
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 110 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
The herbicide, Scepter ® , whose active principle is imazaquin, is commonly used in soybean farming to combat wide-leaf weeds. The basidiomycete, Pleurotus ostreatus, and the ascomycete, Botryosphaeria rhodina, were evaluated for their growth and laccase production when cultured on basal media containing Scepter ® . Both fungi could grow on the herbicide when cultivated in solid and submerged liquid culture in the presence of Scepter ® at concentrations of 0 -6% (v/v) for P. ostreatus, and up to 0 -50% (v/v) for B. rhodina, and in each case produced laccases when assayed against ABTS [2,2 1azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)] and 2,6-dimethoxyphenol. P. ostreatus could tolerate up to 6% of Scepter ® before it became toxic to the fungus, while in the case of B. rhodina, 50% (v/v) Scepter ® was the highest amount that supported grow, and laccase activity was detectable up to 25% (v/v). An inverse relationship existed between the level of Scepter ® in the culture medium that supported fungal growth and laccase production. Analysis of the results showed that the fungi studied presented different behaviour towards Scepter ® in the culture environment.