Unilateral cryptorchism produced by experimental retention of one testis in the abdomen of the mouse results in a rapid decrease in the weight of that testis. At the same time the specific activity of lactate dehydrogenase (LDH) decreases to 70% of the normal value. The sperm-specific isozyme, LDH-X
The effect of environment on lactate dehydrogenase isozymes of cultured somatic cells
โ Scribed by Richard M. Cribbs; Edward S. Kline
- Publisher
- John Wiley and Sons
- Year
- 1971
- Tongue
- English
- Weight
- 511 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
โฆ Synopsis
The lactate dehydrogenases of HeLa cells grown in an anaerobic environment with either glucose or L-lactate have a higher ratio of A to B subunits than do the same cells grown in an aerobic environment. This ratio is decreased when the anaerobic environment is made aerobic during cell growth, or when the cells are cultured for the entire growth period in aerobic conditions.
๐ SIMILAR VOLUMES
Early studies, using kinetic methods, suggested that the isozyme pattern of lactate dehydrogenase in various cells oscillated with time. More recent electrophoretic studies on murine erythroleukaemic cells (which exhibit only one isozyme) indicated very high frequency variations (period 2min or less
The effects of eight cyclodextrins (CDs) on the stability of freeze-dried lactate dehydrogenase (LDH) were investigated. Five low-molecular weight saccharides were used as a reference. In the samples without saccharides, LDH activity was decreased by freeze-drying. The LDH activities of the samples
The effects of cold acclimation on the activity levels of creatine kinase, lactate dehydrogenase and lactate dehydrogenase isoenzymes in various tissues/ organs of the rat (Rattus norvegicus) were investigated. Male Sprague-Dawley rats were divided into two groups. One group was housed at 4+/-1 degr
Growth of KB cells was inhibited by both spermine and spermidine, but the inhibition is reduced in conditioned medium. The amount of spermine required for 50% inhibition of plating varied according to the type of serum used with medium 199 (calf, fetal bovine, and horse; 0.55, 0.9, and 24 pg/ml resp