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Effects of various cyclodextrins on the stability of freeze-dried lactate dehydrogenase

โœ Scribed by Junya Iwai; Noriko Ogawa; Hiromasa Nagase; Tomohiro Endo; Thorsteinn Loftsson; Haruhisa Ueda

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
99 KB
Volume
96
Category
Article
ISSN
0022-3549

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โœฆ Synopsis

The effects of eight cyclodextrins (CDs) on the stability of freeze-dried lactate dehydrogenase (LDH) were investigated. Five low-molecular weight saccharides were used as a reference. In the samples without saccharides, LDH activity was decreased by freeze-drying. The LDH activities of the samples with 2-hydroxypropylated CDs (HP-CDs) such as 2-hydroxypropyl-b-cyclodextrin (HP-b-CD) showed only a small decrease and were more effective protein stabilizers than low-molecular weight saccharides. HP-b-CD more effectively stabilized LDH than trehalose, at a sugar concentration of less than 1.0 w/w% and furthermore, a close relationship existed between the highly stabilizing effect and the degree of substitution of polar substituents.

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