EFFECTS OF COLD ACCLIMATION ON THE ACTIVITY LEVELS OF CREATINE KINASE, LACTATE DEHYDROGENASE AND LACTATE DEHYDROGENASE ISOENZYMES IN VARIOUS TISSUES OF THE RAT

✍ Scribed by S.E. Terblanche; T.C. Masondo; W. Nel

• Publisher: Elsevier Science
• Year: 1998
• Tongue: English
• Weight: 215 KB
• Volume: 22
• Category: Article
• ISSN: 1065-6995
• DOI: 10.1006/cbir.1998.0310

✦ Synopsis

The effects of cold acclimation on the activity levels of creatine kinase, lactate dehydrogenase and lactate dehydrogenase isoenzymes in various tissues/ organs of the rat (Rattus norvegicus) were investigated. Male Sprague-Dawley rats were divided into two groups. One group was housed at 4+/-1 degrees C (experimental group) and the other at 24+/-1 degrees C (control group) for six months. The rats were housed in single cages and had access to food and water ad libitum. The tissues/organs investigated were heart, liver, lung, kidney, gastrocnemius muscle and interscapular brown adipose tissue as well as serum. With the exception of lung, (which showed a decrease of 24%) total creatine kinase activity levels were significantly increased (P< 0.05) in all the tissues/organs investigated (17-51%) as well as serum (34%), in cold acclimated animals. Cold acclimation also resulted in significantly increased (P< 0.05) activity levels of lactate dehydrogenase in all the tissues/organs investigated (14-24%) as well as serum (35%). Cold exposure resulted in an increase of the activity levels of all the detectable isoenzymes of lactate dehydrogenase, although not always significant, in all the tissues/organs investigated as well as serum. The M(4)tetramer of lactate dehydrogenase was the only detectable isoenzyme in serum.