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INDEPENDENT HIGH-FREQUENCY OSCILLATIONS IN THE AMOUNTS OF INDIVIDUAL ISOZYMES OF LACTATE DEHYDROGENASE IN HL60 CELLS

✍ Scribed by G.M.N. FERREIRA; K.D. HAMMOND; D.A. GILBERT

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 310 KB
Volume: 20
Category: Article
ISSN: 1065-6995
DOI: 10.1006/cbir.1996.0079

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✦ Synopsis

Early studies, using kinetic methods, suggested that the isozyme pattern of lactate dehydrogenase in various cells oscillated with time. More recent electrophoretic studies on murine erythroleukaemic cells (which exhibit only one isozyme) indicated very high frequency variations (period 2min or less) in the amount of the lone active isozyme. We now show that in HL60 cells, the activity stain intensities of the two major isozyme bands both oscillate but the temporal variations are distinct. As with other cellular rhythms, each of the two periodicities seem to be modulated in cyclic fashion with respect to period, amplitude and mean levels, the periods of both the primary and modulating rhythms being of the order of 10–15min or probably much less.

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