The chemical synthesis of high specific-activity [35S]adenosylhomocysteine
✍ Scribed by Peter H. Stern; Robert M. Hoffman
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 426 KB
- Volume
- 158
- Category
- Article
- ISSN
- 0003-2697
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📜 SIMILAR VOLUMES
A simple procedure for synthesizing [32P]pyrophosphate with high specific activity (about 100 Ci/mmol) is described. [32P]Pyrophosphate was formed by simple dehydration of inorganic [3\*P]phosphate and subsequently purified by DEAE-Sephadex A-25 chromatography. The yield was 20-40%.
## Abstract Lewis acid‐assisted sulfonylation of anisole with [^35^S]methanesulfonyl chloride afforded high specific activity aryl [^35^S]sulfones. Demethylation and treatment with triflic anhydride gave the versatile [^35^S]triflate **1** in good overall yields. The [^35^S]sulfone triflate could b
## Abstract Prenylated cysteine analogs, which mimic the prenylated cysteine residue of prenylated GTP‐binding proteins (G‐proteins), have been used in a variety of contexts for the study of prenylated G‐protein behavior. In earlier work in this area, we prepared the photoactive analog [^35^S]**4**
## Abstract Unprotected deoxyadenosine **1** was treated with an excess of phosphorus acid and stoichiometric proportions of __N__, __N__′‐di‐__p__‐tolylcarbodiimide in anhydrous pyridine to give deoxyadenosine‐5′‐monophosphite **2**. The latter was activated with trimethylsilyl chloride followed b
The title compound has been prepared by two different approaches. The first involved a five-stage "hot" synthesis.where the tritiation step was selective debromination of a cyclic Fbromo-a-&unsaturated ketone. double bond was protected by formal aromatisation. this intermediate was determined by pro