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Chemical synthesis of [32P]pyrophosphate with high specific activity

✍ Scribed by Kit S. Lam; Charles B. Kasper

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 445 KB
Volume: 108
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90583-7

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✦ Synopsis

A simple procedure for synthesizing [32P]pyrophosphate with high specific activity (about 100 Ci/mmol) is described. [32P]Pyrophosphate was formed by simple dehydration of inorganic [3*P]phosphate and subsequently purified by DEAE-Sephadex A-25 chromatography. The yield was 20-40%.

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