Enzymatic synthesis of [α32P]ATP of high specific activity
✍ Scribed by Kazumitsu Kihara; Hisayuki Nomiyama; Makoto Yukuhiro; Jun-Ichiro Mukai
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 126 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A simple procedure for synthesizing [32P]pyrophosphate with high specific activity (about 100 Ci/mmol) is described. [32P]Pyrophosphate was formed by simple dehydration of inorganic [3\*P]phosphate and subsequently purified by DEAE-Sephadex A-25 chromatography. The yield was 20-40%.
An enzymatic method for the synthesis of [beta-32P]ADP from [gamma-32P]ATP is described. This substrate is required for the assay of ADPase and is not commercially available. The method described results in a preparation of [beta-32P]ADP of high purity with a yield of approximately 40% the theoretic
We found that inorganic compounds labelled with 32P such as PzSj, PCI3,PSC13,POC13, and H3P04 can be obtained by a newsimplemethod, withgood yieldsand a highspecificactivity. The carrier free 3nP resulting through the nuclear reaction 32S(n,p)32P from elementary sulphur was concentrated on red phosp