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Enzymatic synthesis of [α32P]ATP of high specific activity

✍ Scribed by Kazumitsu Kihara; Hisayuki Nomiyama; Makoto Yukuhiro; Jun-Ichiro Mukai


Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
126 KB
Volume
75
Category
Article
ISSN
0003-2697

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📜 SIMILAR VOLUMES


Chemical synthesis of [32P]pyrophosphate
✍ Kit S. Lam; Charles B. Kasper 📂 Article 📅 1980 🏛 Elsevier Science 🌐 English ⚖ 445 KB

A simple procedure for synthesizing [32P]pyrophosphate with high specific activity (about 100 Ci/mmol) is described. [32P]Pyrophosphate was formed by simple dehydration of inorganic [3\*P]phosphate and subsequently purified by DEAE-Sephadex A-25 chromatography. The yield was 20-40%.

Enzymatic synthesis of [β-32P]ADP using
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An enzymatic method for the synthesis of [beta-32P]ADP from [gamma-32P]ATP is described. This substrate is required for the assay of ADPase and is not commercially available. The method described results in a preparation of [beta-32P]ADP of high purity with a yield of approximately 40% the theoretic

New possibilities to obtain 32P-labelled
✍ P. I. Bebeşel; C. N. Turcanu 📂 Article 📅 1966 🏛 John Wiley and Sons 🌐 French ⚖ 194 KB

We found that inorganic compounds labelled with 32P such as PzSj, PCI3,PSC13,POC13, and H3P04 can be obtained by a newsimplemethod, withgood yieldsand a highspecificactivity. The carrier free 3nP resulting through the nuclear reaction 32S(n,p)32P from elementary sulphur was concentrated on red phosp