✦ LIBER ✦

Enzymatic synthesis of [β-32P]ADP using adenylate kinase and [γ-32P]ATP

✍ Scribed by Janet M. Dawson; Neil D. Cook; Timothy J. Peters

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 247 KB
Volume: 149
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(85)90600-1

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✦ Synopsis

An enzymatic method for the synthesis of [beta-32P]ADP from [gamma-32P]ATP is described. This substrate is required for the assay of ADPase and is not commercially available. The method described results in a preparation of [beta-32P]ADP of high purity with a yield of approximately 40% the theoretical obtainable.

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