Specific activity of P32 in cell constituents of rabbit liver
โ Scribed by Marshak, Alfred ;Calvet, Fernando
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1949
- Tongue
- English
- Weight
- 244 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0095-9898
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๐ SIMILAR VOLUMES
A simple procedure for synthesizing [32P]pyrophosphate with high specific activity (about 100 Ci/mmol) is described. [32P]Pyrophosphate was formed by simple dehydration of inorganic [3\*P]phosphate and subsequently purified by DEAE-Sephadex A-25 chromatography. The yield was 20-40%.
We found that inorganic compounds labelled with 32P such as PzSj, PCI3,PSC13,POC13, and H3P04 can be obtained by a newsimplemethod, withgood yieldsand a highspecificactivity. The carrier free 3nP resulting through the nuclear reaction 32S(n,p)32P from elementary sulphur was concentrated on red phosp
The heparin-releasable neutral lipase (EC 3.1.1.3) from rat liver is inactivated by the common preparations of collagenases (EC 3.4.24.3) used for the isolation of liver cells. We show that two collagenases purified from Clostridium histolyticum allow both the complete preservation of this lipolytic