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Synthesis and purification of [1-32P]fructose-1,6-bisphosphate with high specific radioactivity

✍ Scribed by Ronald W. McClard

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
307 KB
Volume
96
Category
Article
ISSN
0003-2697

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✦ Synopsis

The straightforward, rapid synthesis and purification of radiochemically pure [ I-JzP]fructose-1,6-bisphosphate starting from radioactively labeled inorganic phosphate is described. The product has a relatively high specific radioactivity (2 x lo5 Ci/mol). Using this method, carrier-free [ 1-32P]fructose-l,6-bisphosphate could be obtained. The use of [l-32P]fructose-1,6-bisphosphate in the assay of fructose bisphosphatase is illustrated.

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