Synthesis of deoxyadenosine-5′-(35S)-thiomonophosphate [dAMP(35S)] of high specific activity – a key intermediate for the synthesis of Sp-deoxyadenosine-5′-(alpha;-35S)-thiotriphosphate [Sp-dATP (α-35S)]
✍ Scribed by K. M. Mathew; S. Ravi; C. V. Sontakke; H. Chander
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- French
- Weight
- 107 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0022-2135
- DOI
- 10.1002/jlcr.531
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✦ Synopsis
Abstract
Unprotected deoxyadenosine 1 was treated with an excess of phosphorus acid and stoichiometric proportions of N, N′‐di‐p‐tolylcarbodiimide in anhydrous pyridine to give deoxyadenosine‐5′‐monophosphite 2. The latter was activated with trimethylsilyl chloride followed by sulphurisation with elemental ^35^S (specific activity>1000 Ci/mmol) in toluene solution to give deoxyadenosine‐5′‐(^35^S)‐thiomonophosphate [dAMP(^35^S)] 3. Enzymatic conversion of deoxyadenosine‐5′‐(^35^S)‐thiomonophosphate to Sp‐deoxyadenosine‐5′‐(α‐^35^S)‐thiotriphosphate [Sp‐dATP (α‐^35^S)] 5 was carried out following a standard reaction protocol. Copyright © 2001 John Wiley & Sons, Ltd.