A method for the enzymatic synthesis of [1-14 C-N-acetyl, P 18 O 2 ] cytidine monophosphate neuraminic acid (CMP-NeuAc) is described. Central to the synthesis of [1-14 C-N-acetyl, P 18 O 2 ]CMP-NeuAc was the enzymatic preparation of [g-P 18 O 3 ]ATP for use in a reaction with uridine kinase and cyti
The chemical-enzymatic synthesis of a carbon glycoside of N-acetyl neuraminic acid
โ Scribed by Jon O. Nagy; Mark D. Bednarski
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- French
- Weight
- 278 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
V = 1078.3(5) x lo6 pm3, psalsd = 1.089 g ~m -~, Z = 2, crystal size 0.3 x 0.3 x 0.9 mm3, 173 K, Cu,,, radiation, Enraf-Nonius CAD4, scan width 2.25 <8 < 76.5",4202 symmetry-independent and significant reflections [IF1 >3u(F)] structure solution by direct methods (SHELXS-86 [6]) supplemented by diff
N-Acetylneuraminic acid (1) can be transformed into the methyl a-u-ketoside 2 which, by reaction with methanesulfonyl chloride, yields the corresponding 4-0 mesylate 3 and the 4.7-di-0 -mesylate 4 as a by-product. Compound 3 reacts with Nal giving the 4-dcoxy-4-iodo compound 5 with cquatorial orient