## Abstract Application of the phosphorylating agent 2βchlorophenylβ0,0βbis(1βhydroxybenzotriazolyl)βphosphate enabled us to synthesize DNA fragments (e.g., dβAGGATCCT and dβGTTCGG) on a polystyrene solid support:
Synthesis of DNA fragments by the hydroxybenzotriazole phosphotriester approach
β Scribed by J.E. Marugg; M. Tromp; P. Jhurani; C.F. Hoyng; G.A. Van Der Marel; J.H. Van Boom
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- French
- Weight
- 739 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0040-4020
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## Abstract The synthesis of a total of twelve DNA fragments on the solid supports cellulose or polystyrene via a phosphotriester approach is described. Dimer and trimer blocks, protected at the 5β²βend with the levulinoyl group, were used for chain extension in the presence of the activating agent
The present phosphotriester strategy for the (solution-phase) synthesis of the 3'-5' internucleotide bond of DNA is basically a two-step process' (Fig. ). We have modified and simplified this process by coupling together these up-to-now generally uncoupled reactions\* into one continuous and abbrevi
Most of the published work on the chemical synthesis of oligonucleotides\* has involved the us8 of intermediates with unprotected lnternucleotide phosphodiester linkages. Such intermediates can usually be isolated only on a comparatively small scale and are sometimes difficult to obtain pure. Furthe