Large scale H.E.L.P. synthesis of oligodeoxynucleotides by the hydroxybenzotriazole phosphotriester approach.
β Scribed by Francesco P.Colonna; Carlo L.Scremin; Gian M.Bonora
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- French
- Weight
- 230 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0040-4039
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π SIMILAR VOLUMES
## Abstract Application of the phosphorylating agent 2βchlorophenylβ0,0βbis(1βhydroxybenzotriazolyl)βphosphate enabled us to synthesize DNA fragments (e.g., dβAGGATCCT and dβGTTCGG) on a polystyrene solid support:
Most of the published work on the chemical synthesis of oligonucleotides\* has involved the us8 of intermediates with unprotected lnternucleotide phosphodiester linkages. Such intermediates can usually be isolated only on a comparatively small scale and are sometimes difficult to obtain pure. Furthe
4,6-Dinitro-l-(mesitylene-2-sulphonyloxy)-benzotriazole [DMBT, (@)I, l-(mesitylene-2-sulphonyloxy)-4-nitro-6-trifluoromethylbenzotriazole (@) and 1-(mesitylene-2-sulphonyl)-4nitro-1,2,3-triazole [iso-MSNT, (211 are more reactive condensing agents in the phosphotriester approach to oligonucleotide sy