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Synthesis of DNA on a solid support by the hydroxybenzotriazole phosphotriester approach

✍ Scribed by J. E. Marugg; G. A. van der Morel; E. de Vroom; D. Bosch; J. H. van Boom

Publisher: Elsevier Science
Year: 2010
Tongue: English
Weight: 159 KB
Volume: 101
Category: Article
ISSN: 0165-0513
DOI: 10.1002/recl.19821011109

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✦ Synopsis

Abstract

Application of the phosphorylating agent 2‐chlorophenyl‐0,0‐bis(1‐hydroxybenzotriazolyl)‐phosphate enabled us to synthesize DNA fragments (e.g., d‐AGGATCCT and d‐GTTCGG) on a polystyrene solid support:

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