✦ LIBER ✦

Simplification of DNA synthesis by the phosphotriester method

✍ Scribed by P. Cashion; K. Porter; T. Cadger; G. Sathe; T. Tranquilla; H. Notman; E. Jay

Publisher: Elsevier Science
Year: 1976
Tongue: French
Weight: 285 KB
Volume: 17
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(00)93105-8

No coin nor oath required. For personal study only.

✦ Synopsis

The present phosphotriester strategy for the (solution-phase) synthesis of the 3'-5' internucleotide bond of DNA is basically a two-step process' (Fig. ). We have modified and simplified this process by coupling together these up-to-now generally uncoupled reactions* into one continuous and abbreviated reaction sequence (Fig. ). These modifications lead to an increase in the overall yield to near quantitative as well as to a decrease in the working time by at least a factor of 4.

📜 SIMILAR VOLUMES

Synthesis of DNA fragments by the hydrox

Synthesis of DNA fragments by the hydroxybenzotriazole phosphotriester approach

✍ J.E. Marugg; M. Tromp; P. Jhurani; C.F. Hoyng; G.A. Van Der Marel; J.H. Van Boom 📂 Article 📅 1984 🏛 Elsevier Science 🌐 French ⚖ 739 KB

Synthesis of DNA on a solid support by t

Synthesis of DNA on a solid support by the hydroxybenzotriazole phosphotriester approach

✍ J. E. Marugg; G. A. van der Morel; E. de Vroom; D. Bosch; J. H. van Boom 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 159 KB

## Abstract Application of the phosphorylating agent 2‐chlorophenyl‐0,0‐bis(1‐hydroxybenzotriazolyl)‐phosphate enabled us to synthesize DNA fragments (e.g., d‐AGGATCCT and d‐GTTCGG) on a polystyrene solid support:

Block synthesis of oligonucleotides by t

Block synthesis of oligonucleotides by the phosphotriester approach

✍ N.J. Cusack; C.B. Reese; J.H. van Boom 📂 Article 📅 1973 🏛 Elsevier Science 🌐 French ⚖ 261 KB

Most of the published work on the chemical synthesis of oligonucleotides\* has involved the us8 of intermediates with unprotected lnternucleotide phosphodiester linkages. Such intermediates can usually be isolated only on a comparatively small scale and are sometimes difficult to obtain pure. Furthe

Phosphotriester synthesis of DNA fragmen

Phosphotriester synthesis of DNA fragments on cellulose and polystyrene solid supports

✍ G. A. van der Marel; J. E. Marugg; E. de Vroom; G. Wille; M. Tromp; C. A. A. van 📂 Article 📅 2010 🏛 Elsevier Science 🌐 English ⚖ 922 KB

## Abstract The synthesis of a total of twelve DNA fragments on the solid supports cellulose or polystyrene via a phosphotriester approach is described. Dimer and trimer blocks, protected at the 5′‐end with the levulinoyl group, were used for chain extension in the presence of the activating agent

Solid phase synthesis of nonadecathymidy

Solid phase synthesis of nonadecathymidylic acid by the phosphotriester approach

✍ Ken-ichi Miyoshi; Keiichi Itakura 📂 Article 📅 1979 🏛 Elsevier Science 🌐 French ⚖ 206 KB

Synthesis of phosphorodithioate DNA by t

Synthesis of phosphorodithioate DNA by the H-phosphonothioate method

✍ Peter H. Seeberger; Marvin H. Caruthers; Danute Bankaitis-Davis; Graham Beaton 📂 Article 📅 1999 🏛 Elsevier Science 🌐 French ⚖ 863 KB

A novel method for the efficientsynthesis phosphorodithioate DNA using H-phosphonothioate synthons is described. The 5'-DMT-nucleoside H-phosphonothioate monomers were prepared in two high yielding steps from commercially available phosphoramidites. This strategy for the synthesis of Hphosphonothioa