Synthesis of 3′-functionalized oligonucleotides on a single solid support
✍ Scribed by Jari Hovinen; Andrei Guzaev; Alex Azhayev; Harri Lönnberg
- Book ID
- 111714377
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- French
- Weight
- 274 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
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Michaelis-Arbusov chemistry was used to prepare O,S-dialkyl Y-O-nucleosidyl phosphorothiolate triesters in solution and attached to CPG. The support-bound nucleoside was utilised in the synthesis of a pentaribonucleotide that was fully deprotected on the support. Subsequent ~'eatment with a buffere
## Abstract Oligonucleotides containing terminal phosphate groups serve as useful intermediates for different applications in molecular and cell biology, as well as for diagnostic purposes. The chemical synthesis of these derivatives has been an important topic of oligonucleotide research. We repor