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A novel solid support for synthesis of 2′,3′-cyclic phosphate terminated oligonucleotides

✍ Scribed by Joseph S Vyle; Nicholas H Williams; Jane A Grasby

Publisher
Elsevier Science
Year
1998
Tongue
French
Weight
293 KB
Volume
39
Category
Article
ISSN
0040-4039

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✦ Synopsis

Michaelis-Arbusov chemistry was used to prepare O,S-dialkyl Y-O-nucleosidyl phosphorothiolate triesters in solution and attached to CPG.

The support-bound nucleoside was utilised in the synthesis of a pentaribonucleotide that was fully deprotected on the support. Subsequent ~'eatment with a buffered solution of iodine cleaved the RNA l~om the CPG with concomitant formation of a terminal 2',Y-cyclic phosphate.

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