Coniine-15N was obtained by the reductive amination of 5-0x0octanal using sodium cyanoborohydride and a m m o n i ~m -~~N bromide. Nornicotine-l'-15N resulted from a similar reductive aminution of 4-oxo-4-(3'-pyridyl)butanal. Merhybtion with formaldehyde and ,formic acid afforded n i ~o t i n e -I '
Synthesis of [1′-15N]-biotin
✍ Scribed by M.-L. Lee; S. Berger
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- French
- Weight
- 275 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
Racemic [1′‐^15^N]‐biotin was synthesized from [^15^N]‐glycine as starting material in 7 steps. The overall yield was 13%.
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## Abstract Specifically, ^15^N‐enriched L‐histidines have been prepared. The labelling methodology involves introduction of labels in its precursor 1‐benzyl‐5‐hydroxy methyl imidazole, which is converted into L‐histidine via the Schöllkopf method. The procedure allows the preparation of the interm