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Improved specific Synthesis of [1′-15N]- and [3′-15N]L-histidine

✍ Scribed by C. Soede-Huijbregts; M. van Laren; F. B. Hulsbergen; J. Raap; J. Lugtenburg

Publisher: John Wiley and Sons
Year: 2001
Tongue: French
Weight: 123 KB
Volume: 44
Category: Article
ISSN: 0022-2135
DOI: 10.1002/jlcr.507

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✦ Synopsis

Abstract

Specifically, ^15^N‐enriched L‐histidines have been prepared. The labelling methodology involves introduction of labels in its precursor 1‐benzyl‐5‐hydroxy methyl imidazole, which is converted into L‐histidine via the Schöllkopf method. The procedure allows the preparation of the intermediates and finally histidine with high ^15^N enrichment (99%) at each position, in 29% overall yield starting with ^15^NH~4~Cl and 56% with KSC^15^N, respectively. Copyright © 2001 John Wiley & Sons, Ltd.

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