Synthesis and Stability of Novel Terminal Phosphate-Labeled Nucleotides
✍ Scribed by Reynolds, Bambi; Miller, Rachel; Williams, John; Anderson, Jon
- Book ID
- 118166055
- Publisher
- Taylor and Francis Group
- Year
- 2008
- Tongue
- English
- Weight
- 482 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0732-8311
No coin nor oath required. For personal study only.
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## Abstract Treatment of adenosine 5′‐phosphate 1‐oxide with base gave 5‐amino‐1‐β‐ D‐ribofuranosylimidazole‐4‐carboxamidoxime 5′‐ phosphate. Catalytic reduction of this amidoxime gave the versatile intermediate, 5‐amino‐1‐β‐D‐ribofuranosylimidazole‐ 4‐carboxamidine 5′‐phosphate. Condensation of th
tedious task. It requires isotopically labeled nucleo-UTP, labeled with 15 N and 13 C (at all carbon atoms of sides for solid-phase synthesis or the corresponding the ribose moiety), was obtained enzymatically from NTPs for in vitro transcription methods. A variety of [ 15 N]uracil and [ 13 C 6 ]glu
I4C-Labeled inosine 5'-phosphate was synthesized by direct phosphorylation of i n 0 s i n e -8 -~~C with phosphoryl chloride followed by hydrolysis of the resulting phosphorodichloridate. Isolation and recrystallization o f its disodium salt afforded pure material in 66.8% overall chemical yield.