Selective Phosphate Protection: A Novel Synthesis of Double-Labeled Oligonucleotides
β Scribed by Guzaev, Andrei P.; Manoharan, Muthiah
- Book ID
- 126019043
- Publisher
- American Chemical Society
- Year
- 2001
- Tongue
- English
- Weight
- 59 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1523-7060
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π SIMILAR VOLUMES
The utility of the 2-(arylcarbamoyloxy)ethyl group for protection of internucleosidic phosphate linkages in oligonucleotide synthesis was studied. Of the three protecting groups tested, the 2-[(1-naphthyl)carbamoyloxy]ethyl demonstrated high coupling yields, favorable deprotection kinetics and the h
Michaelis-Arbusov chemistry was used to prepare O,S-dialkyl Y-O-nucleosidyl phosphorothiolate triesters in solution and attached to CPG. The support-bound nucleoside was utilised in the synthesis of a pentaribonucleotide that was fully deprotected on the support. Subsequent ~'eatment with a buffere