✍ Scribed by Anne-Marie Gilles; Ioan Cristea; Nicolae Palibroda; Ida Hilden; Kaj Frank Jensen; Robert S. Sarfati; Abdelkader Namane; Joël Ughetto-Monfrin; Octavian Bârzu
No coin nor oath required. For personal study only.
tedious task. It requires isotopically labeled nucleo-UTP, labeled with 15 N and 13 C (at all carbon atoms of sides for solid-phase synthesis or the corresponding the ribose moiety), was obtained enzymatically from NTPs for in vitro transcription methods. A variety of [ 15 N]uracil and [ 13 C 6 ]glucose. Eleven enzymes and suit-chemical or enzymatic methods for uniform or site-speable substrates reconstituted a metabolic pathway in cific 15 N/ 13 C labeling of nucleosides or nucleotides were which glucose was first transformed to 5-phosphoriboproposed over the past years (7-10). Nikonowicz et al. syl-1-pyrophosphate. The latter compound plus uracil (11) and Batey et al. (12) recently described procedures yielded UMP in a second step by the reaction catalyzed for preparation of uniformly labeled NTPs from Escheby uracil phosphoribosyltransferase. UMP was subserichia coli or Methylophilus methylotrophys grown on quently phosphorylated to the corresponding di-and [ 15 N]ammonium sulfate and [ 13 C 6 ]glucose or [ 13 C]triphosphate. ATP, required for five phosphorylation methanol, respectively, with an overall yield (i.e., the reactions, was regenerated from creatine phosphate, molar ratio of NTPs/glucose or methanol consumed) whereas NADP / necessary for the oxidation of glucose between 3 and 4%.
📜 SIMILAR VOLUMES
## Abstract Synthetic methods leading to nucleosides and deoxynucleosides specifically labelled with ^13^C and ^15^N are reviewed. The paper covers labelling the sugar part of the molecule (with ^13^C) and the aglycone part (both with ^13^C and ^15^N). Synthetic methods for labelling at every relev
## Abstract Anandamide (Figure 1a) (arachidonyl ethanolamide, AEA), (5Z,8Z,11Z,14Z)‐N‐(2‐hydroxyethyl)‐5,8,11,14‐Eicosatetraenamide, is an endogenous cannabinoid ligand possessing important biological activity. The conformation of AEA in its native receptor binding environment is particularly of in
Labeled derivatives of N-methylolacrylamide (NMA) including 15 N-NMA, 13 C-NMA, and 13 C, 15 N-NMA were synthesized and purified. A required chemical precursor, 15 N-acrylamide, was also prepared. Reported methods for synthesizing unlabeled analogs are noted, and modifications to these methods for a