Peptide nucleic acids (PNAs) are oligonucleotide mimics in which the sugar-phosphate backbone has been replaced by a pseudo-peptide backbone. Among PNA-based molecules, PNA-DNA conjugates characterized by tracts of DNA bound to N and/or C terminus of PNA are very soluble in aqueous media, are able t
Synthesis and DNA Binding Properties of DNA-PNA Chimeras
β Scribed by G. Barone; L. De Napoli; G. Di Fabio; E. Erra; C. Giancola; A. Messere; D. Montesarchio; L. Petraccone; G. Piccialli
- Publisher
- John Wiley and Sons
- Year
- 2004
- Weight
- 53 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0931-7597
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## Abstract Targeting doubleβstranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNAβPNA chimeras. A 16βmer 5β²βDNAβ3β²βpβ(N)PNA(C) has been investigated for its abilit
## Abstract A new synthetic strategy to get the PNAβ3β²DNA linker with the monomethoxytrityl (Mmt) group as temporary protection of the backbone to be used for the synthesis of PNA/DNA chimeras was employed and a convenient strategy to obtain Mmt PNA monomers was developed. The synthetic strategies