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Targeting duplex DNA with DNA-PNA chimeras? Physico-chemical characterization of a triplex DNA-PNA/DNA/DNA

✍ Scribed by L. Petraccone; E. Erra; A. Messere; D. Montesarchio; G. Piccialli; L. De Napoli; G. Barone; C. Giancola

Publisher: Wiley (John Wiley & Sons)
Year: 2004
Tongue: English
Weight: 194 KB
Volume: 73
Category: Article
ISSN: 0006-3525
DOI: 10.1002/bip.10599

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✦ Synopsis

Abstract

Targeting double‐stranded DNA with homopyrimidine PNAs results in strand displacement complexes PNA/DNA/PNA rather than PNA/DNA/DNA triplex structures. Not much is known about the binding properties of DNA‐PNA chimeras. A 16‐mer 5′‐DNA‐3′‐p‐(N)PNA(C) has been investigated for its ability to hybridize a complementary duplex DNA by DSC, CD, and molecular modeling studies. The obtained results showed the formation of a triplex structure having similar, if not slightly higher, stability compared to the same all‐DNA complex. © 2004 Wiley Periodicals, Inc. Biopolymers, 2004

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