Synthesis and affinity purification of β-32P-labeled [γ-S]GTP
✍ Scribed by Anthony E. Reeve; Ru Chih Huang
- Book ID
- 107711923
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 712 KB
- Volume
- 130
- Category
- Article
- ISSN
- 0003-2697
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📜 SIMILAR VOLUMES
An enzymatic method for the synthesis of [beta-32P]ADP from [gamma-32P]ATP is described. This substrate is required for the assay of ADPase and is not commercially available. The method described results in a preparation of [beta-32P]ADP of high purity with a yield of approximately 40% the theoretic
## Abstract O,S‐Dimethyl phosphoroamidothioate has been synthesized with ^32^P and with ^14^C in the methoxy and the S‐methyl group. Phosphorus trichloride was reacted with sulfur giving phosphorrus thiochloride. The latter was reacted with methanol in the presence of acridine and calcium oxide yie
The s y t h e s i s of carbon-14 labeled l-ir-3-ar~,'lI-7f~r~c.q, Icdtosine and i t s hydrochloride salt i s described. i n four steps from bariwn carbonats-'4C, are labeterl in ;ve Z-7 position of the pyrimidine ring.