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The synthesis of 32P and 14C-labelled o,S-dimethyl phosphoroamidothioate

✍ Scribed by J. A. Lubkowitz; D. F. Horler; J. Baruel

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
263 KB
Volume
10
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

O,S‐Dimethyl phosphoroamidothioate has been synthesized with ^32^P and with ^14^C in the methoxy and the S‐methyl group. Phosphorus trichloride was reacted with sulfur giving phosphorrus thiochloride. The latter was reacted with methanol in the presence of acridine and calcium oxide yielding O‐methyl phosphorodichloridothioate. Isomerization at 100°C gave S‐methyl phosphorodichloridothioate. Finally, treatment with methanol and ammonia gave O,S‐dimethyl phosphoroamidothioate. ^32^P‐labelled phosphorus trichloride and ^14^C‐methanol were appropriately introduced to yield the desired compound.

The intermediates and final product were characterized by NMR, mass spectra and TLC. Products were purified by solvent partition (S‐methyl labelled) and anion exchange chromatography (^32^P‐labelled). Radiochemical purity was determined by TLC. Liquid Scintillation counting was used to determine specific activities. The ^32^P‐labelled compound had a specific activity of 163 μCi/mM while the ^14^C‐methoxy and S‐methyl compounds had specific activities of 86 and 68 μCi/mM respectively.

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