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Spectrophotometric Assay Using o-Phthaldialdehyde for Determination of Proteolysis in Milk and Isolated Milk Proteins

✍ Scribed by Church, Frank C.; Swaisgood, Harold E.; Porter, David H.; Catignani, George L.


Book ID
120710433
Publisher
American Dairy Science Association
Year
1983
Tongue
English
Weight
690 KB
Volume
66
Category
Article
ISSN
0022-0302

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The use of the o-phthalaldehyde reaction
✍ Stanley A. Robrish; Christopher Kemp; William H. Bowen πŸ“‚ Article πŸ“… 1978 πŸ› Elsevier Science 🌐 English βš– 618 KB

Alkaline hydrolysis of protein followed by reaction with o-phthalaldehyde has permitted the determination of less than 10 ng of protein fluorometrically. When intact proteins were analyzed with o-phthalaldehyde the reaction was less sensitive. This reaction has been applied to analysis of the protei