✦ LIBER ✦

The use of the o-phthalaldehyde reaction as a sensitive assay for protein and to determine protein in bacterial cells and dental plaque

✍ Scribed by Stanley A. Robrish; Christopher Kemp; William H. Bowen

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 618 KB
Volume: 84
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90500-6

No coin nor oath required. For personal study only.

✦ Synopsis

Alkaline hydrolysis of protein followed by reaction with o-phthalaldehyde has permitted the determination of less than 10 ng of protein fluorometrically. When intact proteins were analyzed with o-phthalaldehyde the reaction was less sensitive. This reaction has been applied to analysis of the protein content of dental plaque and bacterial cells.

METHODS

Bacterial

cultures. The bacteria tested were stock strains normally maintained in this laboratory. These included those identified as Streptococcus

📜 SIMILAR VOLUMES

Use of molecular dynamics data in bioche

Use of molecular dynamics data in biochemistry courses : An amphipathy scale to determine protein α-helix transmembrane segments

✍ Fernanda M. Mazzé; Carlos A. Fuzo; Léo Degrève; Pietro Ciancaglini 📂 Article 📅 2008 🏛 The American Society for Biochemistry and Molecula 🌐 English ⚖ 431 KB 👁 1 views

## Abstract The aim of this manuscript is to explain the application of an amphipathy scale obtained from molecular dynamics simulations and to demonstrate how it can be useful in the protein structure field. It is shown that this scale is easy to be used with the advantage of revealing domains of

Combined measurement of the c-erbb-2 pro

Combined measurement of the c-erbb-2 protein in breast carcinoma tissues and sera is useful as a sensitive tumor marker for monitoring tumor relapse

✍ Kokichi Sugano; Mineko Ushiama; Takashi Fukutomi; Hitoshi Tsuda; Takashi Kitoh; 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 222 KB 👁 2 views

Polyaniline as a cathode for O2 reductio

Polyaniline as a cathode for O2 reduction—Kinetics of the reaction with H2O2 and use of the polymer in a model H2O2 fuel cell

✍ Lioudmila Doubova; Giuliano Mengoli; Marco M. Musiani; Sergio Valcher 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 525 KB

Oxygen reduction at a polyaniline cathode occurs in aqueous sulfuric acid through a chemica-electrochemical route which involves the intermediate oxidation of leucoemeraldine to emetaldine with the formation of H,O,. This paper specifically deals with the conversion of leucoemeraldine to emeraldine

Plasma (1→3)-β-D-glucan assay and immuno

Plasma (1→3)-β-D-glucan assay and immunohistochemical staining of (1→3)-β-D-glucan in the fungal cell walls using a novel horseshoe crab protein (T-GBP) that specifically binds to (1→3)-β-D-glucan

✍ Hiroshi Tamura; Shigenori Tanaka; Teruo Ikeda; Taminori Obayashi; Yohichi Hashim 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 95 KB 👁 1 views

A highly sensitive enzyme-linked immunosorbent assay specific to (1→3)-β-Dglucans (GBP-ELISA) has been developed using a novel (1→3)-β-D-glucan-binding protein (T-GBP), which was purified from the amebocyte lysate of the Japanese horseshoe crab, Tachypleus tridentatus. This method allowed quantitati

Acute and Chronic Toxicity of Veratrum V

Acute and Chronic Toxicity of Veratrum Viride††The Veratrum viride studied was the whole-powdered drug marketed by Irwin, Neisler & Co. under the name, Vertavis. The work reported herein was supported by a grant from this firm. The batch of powder used in these experiments was stated by the manufacturer to have been assayed biologically by the Craw method on Daphnia magna. One Craw unit of the material studied was stated to be 15.6mg. With regard to this assay, Mr. R. O. Craw has been so kind as to allow us to publish a brief explanation: A reference lot of Yerolrum oirid; powder was set-aside as a standard and a 10% w/v aqueous extract was made of this powder. The average median length of life in 50 assays of 1000 daphnia on immersion in the solution was determined to be 2.22 hours. The daphnia were considered dead when the heart was found to be in systolic standstill on microscopic examination. Succeeding lots of powder were assayed and their potency determined by relating the median length of life of daphnia to 2.22 hours, which is one unit. Twenty assays of 10 animals each were used on each lot of powder.‡‡The authors wish to thank Dr. J. F. A.,McManus of the Department of Pathology for a pathologic report on the tissues, and Mr. James Thomas and Mrs. Bobby E. Teague for technical assistance.

✍ Perdue, Adoline S. ;Bell, Frances ;Starnes, W.R. ;Hitchcock, Philip ;Teague, R.S 📂 Article 📅 1950 🏛 Elsevier ⚖ 346 KB 👁 1 views