𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Specific Ligands for the Affinity Chromatography of Cholinergic Proteins

✍ Scribed by Gaetano Riggio; Wolfgang Herbert Hopff; Alfred André Hofmann; Peter Gaudenz Waser

Publisher
John Wiley and Sons
Year
1980
Tongue
German
Weight
571 KB
Volume
63
Category
Article
ISSN
0018-019X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Affinity chromatography of proteins requires a ligand covalently bound to a solid support separated by a spacer of sufficient length. In the specific case of acetyl‐cholinesterase we have reduced the conventional spacer synthesis from five to three steps.

For affinity chromatography of cholinergic proteins the ideal ligand would be acetylcholine which, however, could not be used because it is easily hydrolyzed. We synthesized hydrolysis‐resistent ligands. Different specific ligands were synthesized for the affinity chromatography of serum esterase.

📜 SIMILAR VOLUMES

The design, synthesis and evaluation of
The design, synthesis and evaluation of affinity ligands for prion proteins
✍ Emma N. Soto Renou; Geeta Gupta; Duncan S. Young; Denise V. Dear; Christopher R. 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 339 KB

## Abstract Bifunctional affinity ligands based on a triazine scaffold were rationally designed to target prion protein and shown to bind recombinant prion protein with high affinity and selectivity. The ligands were capable of discriminating between prion protein glycoforms and monomeric and dimer

Quantification of peptide aldehyde ligan
Quantification of peptide aldehyde ligands immobilized for the affinity chromatography of endopeptidases
✍ Pamela M. Dando; Alan J. Barrett 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 484 KB

A method is described for quantification of aldehyde and aldehyde semicarbazone groups attached to an insoluble matrix. Semicarbazones are converted to aldehydes, and the aldehydes are coupled with 4-phenylazoaniline. The excess reagent is washed off, and the remainder then displaced with salicylald