Thin-Layer Affinity Chromatography in Analysis of Protein–Ligand Affinity
✍ Scribed by Mika P.A. Laitinen; Kirsi M. Sojakka; Matti Vuento
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 154 KB
- Volume
- 243
- Category
- Article
- ISSN
- 0003-2697
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📜 SIMILAR VOLUMES
## Abstract Affinity chromatography of proteins requires a ligand covalently bound to a solid support separated by a spacer of sufficient length. In the specific case of acetyl‐cholinesterase we have reduced the conventional spacer synthesis from five to three steps. For affinity chromatography of
Copolymers of N-isopropylacrylamide and Nacryloyl amino acid spacers of varying chain length were synthesized. p-Aminobenzamidine (PABA) was chemically linked to the pendant carboxyl groups of these polymers to obtain thermoprecipitating affinity polymers. The inhibition constant (K i ) of these pol