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Quantification of peptide aldehyde ligands immobilized for the affinity chromatography of endopeptidases

✍ Scribed by Pamela M. Dando; Alan J. Barrett

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
484 KB
Volume
204
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is described for quantification of aldehyde and aldehyde semicarbazone groups attached to an insoluble matrix. Semicarbazones are converted to aldehydes, and the aldehydes are coupled with 4-phenylazoaniline. The excess reagent is washed off, and the remainder then displaced with salicylaldehyde. The quantity of the phenylazoaniline/salicylaldehyde adduct is determined spectrophotometrically, allowing the calculation of the amount of aldehyde or semicarbazone per unit volume of the matrix. Analyses by the new method show that four matrices offered commercially for this type of immobilization differ greatly in coupling capacity and stability of the conjugate under conditions of affinity chromatography.

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