## Abstract An integrated platform consisting of protein separation by CIEF with monolithic immobilized pH gradient (M‐IPG), on‐line digestion by trypsin‐based immobilized enzyme microreactor (trypsin‐IMER), and peptide separation by CZE was established. In such a platform, a tee unit was used not
On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis
✍ Scribed by Lingyi Zhang; Lihua Zhang; Weibing Zhang; Yukui Zhang
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 271 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## On -line immobilized metal affinity chromatography/capillary electrophoresis/electrospray ionization-mass spectrometry (IMAC/CE/ESI-MS) offers selective preconcentration of phosphorylated peptides with identification of the phosphorylated amino acid(s). The preconcentration provides low concent
## Abstract Macroporous polyacrylamide gels (MPAAG) with iminodiacetic acid (IDA) functionality were prepared by (i) chemical modification of polyacrylamide gel, (ii) co‐polymerization of acrylamide with allyl glycidyl ether (AGE) and __N__,__N__′metylene‐__bis__(acrylamide) (MBAAm) followed by cou