𝔖 Bobbio Scriptorium
✦   LIBER   ✦

On-line concentration of peptides and proteins with the hyphenation of polymer monolithic immobilized metal affinity chromatography and capillary electrophoresis

✍ Scribed by Lingyi Zhang; Lihua Zhang; Weibing Zhang; Yukui Zhang


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
271 KB
Volume
26
Category
Article
ISSN
0173-0835

No coin nor oath required. For personal study only.


📜 SIMILAR VOLUMES


Integration of capillary isoelectric foc
✍ Tingting Wang; Junfeng Ma; Guijie Zhu; Yichu Shan; Zhen Liang; Lihua Zhang; Yuku 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 216 KB

## Abstract An integrated platform consisting of protein separation by CIEF with monolithic immobilized pH gradient (M‐IPG), on‐line digestion by trypsin‐based immobilized enzyme microreactor (trypsin‐IMER), and peptide separation by CZE was established. In such a platform, a tee unit was used not

Mapping the phosphorylation sites of pro
✍ Ping Cao; John T. Stults 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 127 KB 👁 2 views

## On -line immobilized metal affinity chromatography/capillary electrophoresis/electrospray ionization-mass spectrometry (IMAC/CE/ESI-MS) offers selective preconcentration of phosphorylated peptides with identification of the phosphorylated amino acid(s). The preconcentration provides low concent

Macroporous polyacrylamide monolithic ge
✍ Fatima Plieva; Beata Bober; Maria Dainiak; Igor Yu. Galaev; Bo Mattiasson 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 239 KB

## Abstract Macroporous polyacrylamide gels (MPAAG) with iminodiacetic acid (IDA) functionality were prepared by (i) chemical modification of polyacrylamide gel, (ii) co‐polymerization of acrylamide with allyl glycidyl ether (AGE) and __N__,__N__′metylene‐__bis__(acrylamide) (MBAAm) followed by cou