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The purification of β-galactosidase-specific polysomes by affinity chromatography

✍ Scribed by Ulrich Melcher


Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
291 KB
Volume
64
Category
Article
ISSN
0003-2697

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Purification of β-glucosidase activities
✍ Julian N. Kanfer; Richard A. Mumford; Srinivasa S. Raghavan; James Byrd 📂 Article 📅 1974 🏛 Elsevier Science 🌐 English ⚖ 312 KB

Bovine spleen p-n-glucosidase, glucosylceramide : ,6-n-glucosidase and glucosylsphingosine: p-n-giueosidase were purified by chromatography on a "gluconate" Sepharose column. Ten other lysosomal acid hydrolases, present in the preparation applied to the column, were absent from the glucosidase frac