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Purification of β-glucosidase activities from bovine spleen by affinity chromatography

✍ Scribed by Julian N. Kanfer; Richard A. Mumford; Srinivasa S. Raghavan; James Byrd

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
312 KB
Volume
60
Category
Article
ISSN
0003-2697

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✦ Synopsis

Bovine spleen p-n-glucosidase, glucosylceramide : ,6-n-glucosidase and glucosylsphingosine:

p-n-giueosidase were purified by chromatography on a "gluconate" Sepharose column. Ten other lysosomal acid hydrolases, present in the preparation applied to the column, were absent from the glucosidase fraction. 206

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