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Purification of malic enzyme from bovine heart mitochondria by affinity chromatography

✍ Scribed by Trinidad Caldés; Hasmukh R. Fatania; Keith Dalziel

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
394 KB
Volume
100
Category
Article
ISSN
0003-2697

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✦ Synopsis

A simple two-step method for the purification of malic enzyme from bovine heart mitochondria in high yield is described. It consists of successive affinity chromatography steps on immobilized C8-(aminohexyl)-NADP and W-(aminohexyl)-ADP.

The molecular weight estimated by gel filtration of the homogeneous enzyme is 250,000 and the subunit molecular weight by SDS-polyacrylamide gel electrophoresis is 59,000.

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