Using differential dye-ligand chromatography and affinity elution with a substrate analog, 6-phosphogluconate dehydratase (EC 4.2.1.12) has been isolated from extracts of Zymomonas mobilis in a one-step procedure with 50% recovery. The specific activity of freshly isolated enzyme was 245 units mg-1.
โฆ LIBER โฆ
Purification of some tricarboxylic acid cycle enzymes from beef heart using affinity elution chromatography
โ Scribed by Jeffrey R. Davies; Robert K. Scopes
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 708 KB
- Volume
- 114
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Use of differential dye-ligand chromatog
โ
R.K. Scopes; K. Griffiths-Smith
๐
Article
๐
1984
๐
Elsevier Science
๐
English
โ 404 KB
Use of differential dye-ligand chromatog
โ
R.K. Scopes
๐
Article
๐
1984
๐
Elsevier Science
๐
English
โ 449 KB
2-Keto-3-deoxy-6-phosphogluconate aldolase (EC 4.1.2.14) has been isolated from extracts of Zymomonas mobilis using differential dye-ligand chromatography and affinity elution with product/product analog. The one-step procedure gives an enzyme with specific activity 600 units mg-1. Only 1 out of 47