✦ LIBER ✦
Purification of malic enzyme by affinity chromatography on immobilized (N6-(6-aminohexyl)-adenosine 2′,5′-bisphosphate
✍ Scribed by Kwok Kam Yeung; Robert J. Carrico
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 1008 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A simple and rapid method for the purification of NADP-dependent malic enzyme from chicken liver is described. A crude tissue extract was chromatographed on an affinity column containing immobilized W-(6arninohexyl)adenosine 2',5'-bisphosphate. Malic enzyme bound to the ligand and was eluted specifically by a gradient of NADP. Then the enzyme was purified further by gel filtration. The entire procedure requires 50 hr and provides the enzyme in about 40% yield. The purified enzyme migrated as one band during disc electrophoresis in polyacrylamide gel.