✦ LIBER ✦

Purification of pigeon liver malic enzyme by affinity chromatography

✍ Scribed by Jaw-Tang Chang; Gu-Gang Chang

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 336 KB
Volume: 121
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90494-8

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✦ Synopsis

A simple and rapid method for the purification of malic enzyme (EC 1.1.1.40) from pigeon liver is described. Malic enzyme in the crude tissue extract was partially purified by heat treatment, ammonium sulfate fractionation, and DEAE-cellulose chromatography. Final purification was achieved by affinity chromatography on immobilized N6-(6-aminohexyl)-adenosine 2',5'-bisphosphate. Apparently homogeneous enzyme was obtained in 2 days with 54% yield.

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