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One-Step Purification of Bovine Adrenal Glucose-6-phosphate Dehydrogenase by Affinity Chromatography

✍ Scribed by Ernst Bürgisser; Jean-Luc Fauchère

Publisher: John Wiley and Sons
Year: 1976
Tongue: German
Weight: 456 KB
Volume: 59
Category: Article
ISSN: 0018-019X
DOI: 10.1002/hlca.19760590307

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Das konventionelle Reinigungsverfahren von Glucose‐6‐phosphat‐dehydrogenase (G6PDH) (E.C. 1.1.1.49) aus Rinder‐Nebennierenrinde wird durch eine einzige affinitätschromatographische Trennungsstufe ersetzt. Die Anwendung einer mit Nicotinamid‐Adenin‐Dinukleotid‐Phosphat (NADP) substituierten Sepharose ergibt aus dem Rohextrakt eine Protein‐Fraktion, aus welcher nach Einengen die G6PDH unmittelbar kristallisiert. Die Reinheit des Enzyms wird durch analytische Gel‐Elektrophorese sowie durch Vergleich mit schon bestehenden. Literaturdaten geprüft. Vier weitere NADP‐abhängige Dehydrogenasen werden im Eluat identifiziert und partiell gereinigt.

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