✦ LIBER ✦

Purification of a UTP: d-Glucose-1-phosphate uridylyl-transferase from Golgi apparatus of cat liver by affinity chromatography on UTP-agarose

✍ Scribed by G. Azzar; F. Persat; A. Degiuli; R. Got

Publisher: Elsevier Science
Year: 1984
Tongue: English
Weight: 379 KB
Volume: 142
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90499-8

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✦ Synopsis

UDP-glucose pyrophosphorylase from Golgi apparatus solubilized by detergent has been purified 100-fold from microsomes by affinity chromatography on UTP-agarose. The purified enzyme has apparent Mr 270,000 and isoelectric pH 3.9 against 360,000 and 4.2 for soluble enzyme. According to these characteristics, UDP-glucose pyrophosphorylase from Golgi apparatus is different from cytosolic enzyme.