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The purification of yeast glucose 6-phosphate dehydrogenase by dye-ligand chromatography

✍ Scribed by Edward E. Farmer; John S. Easterby

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
280 KB
Volume
141
Category
Article
ISSN
0003-2697

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✦ Synopsis

Glucose 6-phosphate dehydrogenase (EC 1.1.1.39) has been purified to homogeneity from baker's yeast by a simple procedure involving affinity elution from a column of red triazine dye, H-8BN, immobilized to Sepharose 6B. Eight milligrams of homogeneous protein is obtained in 53% yield from 200 g of dried yeast. This represents the first published purification of the enzyme from Saccharomyces Cerevisiae.

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