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Purification by dye-ligand chromatography of an NADP-dependent enzyme, 6-phosphogluconate dehydrogenase from lamb's liver

✍ Scribed by A. Carne

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 249 KB
Volume: 121
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90472-9

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✦ Synopsis

Purification of the NADP'-dependent 6-phosphogluconate dehydrogenase from lamb's liver using dye-ligand chromatography on Matrex Gel Red-A enabled a 2.5fold increase in yield of the enzyme and reduced the preparation time of a preivous scaled-up version of the published small-scale method.

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