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Specific glycolipid antigen(s) in SV40-transformed cell membranes

✍ Scribed by Sandra Ansel; Christian Huet

Publisher
John Wiley and Sons
Year
1980
Tongue
French
Weight
711 KB
Volume
25
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

A glycolipid extract was prepared from an SV40‐transformed^1^ hamster cell line (EH‐SV) according to the Folch partition procedure. The glycolipids from the aqueous layer were incorporated in liposomal membranes composed of lecithin/sphingomyelin/cholesterol (1:1:2 by weight). This liposomal preparation was inoculated in Syrian hamsters to raise immune sera. The sera were absorbed with trypsinized «normal» hamster cells (EH‐N) and tested on various cell lines by the indirect immunofluorescence technique. When used for staining living cells, the immune serum produced a distinct cellsurface fluorescence with SV40‐transformed cell lines regardless of the cell origin (e.g., rat or hamster). No reaction was observed with heterologous Py‐transformed cell lines, spontaneously transformed cells, or sera from non‐immunized hamsters. When used for staining acetone‐fixed cells, the antiglycolipid serum reacted specifically with a thermostable antigen in the nuclear envelope and the cytoplasm of SV40‐transformed cells. The sera lack interfering SV40 T reactivity. The results indicate the presence of related SV40‐specific glycolipid antigen(s) in the plasma membrane, the nuclear membrane and probably other endomembranes of SV40‐transformed cells.

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